WebA Better Way to Normalize Quantitative Western Blots. Total protein normalization using stain-free imaging technology allows normalization across a wider dynamic range. Linearity comparison of stain-free total protein measurement and immunodetection of three housekeeping proteins in 10–50 μg of HeLa cell lysate. WebFluorescent multiplexing enables the quantification of phosphoproteins on a single blot. The Phospho-Specific Mouse Anti-EGF Receptor pSer1047 ( VMA00746) and anti-total Rabbit …
Phospho-Akt (Ser473) Antibody Cell Signaling Technology
WebWestern blot analysis of NFATC1 in serum treated COLO205 whole cell lysates using a Phospho-NFATC1 (Ser172) Polyclonal Antibody (Product # PA5-64696). Product Details Product Specific Information Phospho … WebJul 10, 2024 · This conversion helps to increase the otherwise limited sensitivity of the radioisotope. However, this gain in sensitivity couples with decreased resolution and non … solar renewables
Western blot troubleshooting tips Abcam
WebRabbit anti-phospho-Synaptotagmin (Thr202) FORM This polyclonal antibody is supplied as a 100 µL aliquot in 10 mM Hepes (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% ... On Western blots, it identifies a band at ~60-62 kDa. Antibody specificity was demonstrated by an inhibition assay, as the specific band was blocked by the ... WebThe only difference between the blots is that one membrane is blocked in milk, and the other membrane is blocked in BSA. Clearly, the membrane blocked in BSA has a much higher background. Yet, phospho signal is still strong and clean with the membrane blocked in milk. WebWestern Blotting Protocol (Primary Ab Incubation In BSA) For Western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Products available from Cell Signaling Technology are linked by their respective catalog numbers. A. Solutions and Reagents solar required to generate 1000 kwh per month