Fitc rabbit anti- active caspase-3

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August undefined, 2024

WebWestern blot - Active Caspase-3 Rabbit mAb (A11021) Western blot analysis of extracts of Jukat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution.Jurkat cells … WebCaspases play a crucial role in cell apoptosis or programmed cell death. 70 Effector caspase 3 is responsible for the actual cleavage of cellular components during apoptosis. Hence, in the present study, caspase-3 level was estimated using FITC rabbit anti-active caspase-3 IgG antibody assay kit (BD Biosciences, catalogue no. 559341).

Anti-Caspase-3 antibody (ab4051) Abcam

WebWestern blot analysis of extracts from HCT116 cells (lane 1) or CASP3 knock-out cells (lane 2) using Caspase-3 Antibody #9662 (upper), and α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower). The absence of signal … WebTNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa (1,2) that forms a homotrimer. mTNF-alpha is cleaved by TNF-alpha converting enzyme (TACE) and released in its 157 aa, 17 kDa soluble form (sTNF-alpha) (1-5). Both mTNF-alpha and sTNF-alpha are capable of ... simple chow fun recipe

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WebJan 6, 2024 · caspase-3 Antibody (E-8) is a mouse monoclonal IgG 2a κ caspase-3 antibody, cited in 729 publications, provided at 200 µg/ml. raised against amino acids 1-277 representing the full length precursor form of caspase-3 of human origin. caspase-3 Antibody (E-8) is recommended for detection of caspase-3 and full length procaspase-3 … WebDec 1, 2024 · To identify whether compound 1g exhibits apoptosis, we performed apoptosis assay using Annexin V/FITC, and the fluorescence intensities of FITC-conjugated annexin-V and PI in cells and were analyzed using flow cytometry ().Apoptosis is the critical process for the death of the cancer cells. Upon exposure with compound 1g at IC 50 value, 44.34% … WebMar 22, 2024 · In brief, for active caspase-3 assay, cells were fixed in BD Cytofix/Cytoperm solution, incubated on ice for 20 min, washed with BD Perm/Wash buffer, and then incubated for 30 min at 25°C with FITC rabbit anti-active caspase-3 antibody. rawat weather

caspase-3 Antibody (E-8) SCBT - Santa Cruz Biotechnology

Fitc rabbit anti- active caspase-3

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WebRabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Caspase-3 as the name implies is a member of the cysteine-aspartic acid protease family. Caspase-3 gets cleaved from … WebAug 17, 2024 · Plants are a versatile source of biologically active compounds for anticancer drug discovery ... Primary and secondary antibodies included: rabbit anti-Bcl-2, rabbit anti-poly-ADP-ribose polymerase (PARP), rabbit anti-caspase 3, rabbit anti-AMPK, rabbit anti-mTOR, rabbit anti-Bax, mouse anti-tubulin, anti-rabbit and anti-mouse IgG HRP …

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WebWestern blot - Active Caspase-3 Rabbit mAb (A11021) Western blot analysis of extracts of Jukat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution.Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Webanti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody II. Warnings and Precautions 1. For Research Use Only. Not for use in diagnostic procedures. 2. Reagents contains sodium azide; harmful if swallowed or absorbed through the skin. Sodium azide can

WebHuman Active Caspase-3 Fragment. Validations. None available. Applications. Enzyme-linked immunosorbent assay (ELISA) Immunohistochemistry-frozen tissue (IHC-Frozen) … WebJan 9, 2024 · Caspase-3 expression in HCT-116 cells was quantified through flow cytometry by using the FITC rabbit anti-active caspase-3 IgG antibody. A significant increase in the caspase-3 FITC-MFI was observed in HCT-116 cells treated with AgNPs (1936, 1.5-fold increase) and those treated with cisplatin (44 μg/mL) (4551, 4.5-fold increase), as …

WebAbcam fitc rabbit anti active caspase 3 Fitc Rabbit Anti Active Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

WebSpecificity. Caspase-3 is a member of the interleukin-1 β-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is …

WebSep 23, 2014 · The caspase activity was assessed by the FITC rabbit anti-active caspase-3 (BD Pharmingen, USA). The cells were treated with CuE of 0, 0.625, 1.25 and 2.5 μM for 24 hours. rawaudio bluetooth speakerWebBecton Dickinson fitc conjugated rabbit anti active caspase 3 monoclonal antibody Fitc Conjugated Rabbit Anti Active Caspase 3 Monoclonal Antibody, supplied by Becton … rawaudio bluetooth speaker manualWebAnti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow. Immunocytochemistry Analysis: A … rawat universityWebThese antibodies target Caspase 3 in Human, Mouse, Rat, Non-human primate and Goat samples. Our Caspase 3 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Cell treatment, Knockout and Knockdown to confirm specificity to … rawaudio ffmpegWebProduct name: FITC Rabbit Anti-Active Caspase-3 (51-68654X) (Contd. of page 1) 33.1 · After swallowing If symptoms persist consult doctor. 5 Firefighting measures · Suitable extinguishing agents CO2, ABC multipurpose dry chemical or water spray. Fight larger fires with water spray or alcohol resistant foam. raw audio playerWebCellular detection of the apoptotic markers caspase-3, bcl-2 and Bax activity was determined by FCM employing the fluorescein isothiocyanate conjugated monoclonal antibodies (caspase 3 FITC, Rabbit anti- active caspase 3 cat. No, 559341), (PE Hamster Anti-Mouse Bcl-2 Set Cat No. 556537) and (Bax Monoclonal Antibody (6A7) Cat No. … rawauthentic.comWebFeb 1, 2008 · USA: rabbit anti-active caspase-3 polyclonal antibody (cat. no. 557035), FITC- conjugated rabbit anti-active caspase-3 monoclonal antibody (cat. no. 559341), and PE-conjugated affinity purified ... simple christening message